In order to ensure the quality of its products and the better and more efficient participation in the individual sections of the GENOMILKS research project, in cooperation with EKETA-INEV and after a study, MEVGAL has proceeded to the creation of a new Molecular Biology laboratory at its premises. The equipment of the laboratory, which is state-of-the-art, includes the following:
- Real-Time PCR System
- Real-time Real-time PCR system – Real-time PCR system, including a real-time PCR system.
- Gel imaging system
- Electrophoresis device
- Qubit
- Centrifuge
- Thermal block
- Magnetic stirrer
- Vortex
The primary objective of the laboratory is the detection and identification of the food-borne microorganism Bacillus cereus in samples of raw and fresh milk, which is a useful tool for the decision making of the Quality Department.
In more detail, the procedure followed to achieve the objective is as follows:
The Real-Time PCR system helps to perform a sensitive molecular technique that allows the detection and quantification of DNA in real time. This technique is a significant improvement over traditional detection methods (e.g. growth of the microorganism in a friction cell), as it provides accurate results in a shorter time. It is a useful tool for detecting and monitoring the presence of B. cereus in our dairy products, making its use vital to ensure food quality and hygiene.
The protection chamber for PCR applications is designed to provide a controlled and sterile environment for conducting PCR experiments. It helps to minimise contamination during the testing process, providing more reliable and accurate results.
The gel imaging system and the electrophoresis device contribute to the analysis and visualisation of the results of PCR experiments, allowing comparison of samples with known B. cereus standards.
Through Qubit, a high precision system that allows the measurement of DNA concentration, it will be possible to quantify DNA in samples and thus molecular pathogen detection techniques will be applied with high accuracy.
The use of the centrifuge helps to separate the different components of the sample, while the thermal block helps to ensure the temperature stability of the sample during their preparation.
Finally, the magnetic stirrer and vortex help to achieve appropriate mixing and agitation of the samples.
In conclusion, it is clear that the new molecular laboratory of MEVGAL, through its modern equipment, provides the possibility to carry out highly sensitive processes which will play a catalytic role both in the execution of the individual tasks of the GENOMILKS research programme and in ensuring the quality and hygiene of MEVGAL’s products.
